Cytochalasin b glucose uptake

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WebIn vitro and in vivo study of phloretin-induced apoptosis in human liver cancer cells involving inhibition of type II glucose transporter. Chih Hsiung Wu, Yuan Soon Ho, Chia Yi Tsai, Ying Jan Wang, How Tseng, Po Li Wei, Chia Hwa Lee, Ren Shyan Liu, Shyr Yi Lin. WebRCME cells exhibited stereospecific hexose uptake inhibited by cytochalasin B. Pretreatment of the RCME cells with potassium cyanide or 2,4-dinitrophenol inhibited 2-deoxyglucose uptake but not 3-O-methylglucose transport. A major proportion (30–60%) of the 2-deoxyglucose present in the RCME cells was not phosphorylated.

Cytochalasin B: Inhibition of Glucose and Glucosamine Transport

WebCytochalasin B (1 mg; Sigma-Aldrich; C6762). Add 2 ml DMSO into a vial with 1 mg cytochalasin B (500 μg; 100× CB stock solution). Divide into small tubes (10–20 μl) and store at − 80 °C. • TSA (1 mg; Sigma-Aldrich; T8552). how does kehinde wiley choose his models

Analysis of Insulin-Stimulated Glucose Uptake in

WebApr 23, 2015 · Moreover, cytochalasin B, a nonspecific glucose transport inhibitor, also strongly inhibited the uptake of 14 C-glucose, 14 C-2DG and 2DG-IR in neuronal and astrocytic cultures . WebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ... Web2.2 Assaying Insulin-Stimulated Glucose Uptake 1. Serum-free media: DMEM (with 4.5 g/L glucose, l -glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin (BSA). 2. 2-Deoxyglucose (Sigma) stock: 100 m M (16.4 mg/mL in double-distilled water). Store at 2°–4°C up to 2 wk. 3. photo of age spots

Mechanism of inhibition of human glucose transporter GLUT1 is ... - PNAS

Cytochalasin B - Wikipedia

WebNov 11, 2008 · Non-carrier-mediated uptake was determined in parallel wells containing 10 μmol/L cytochalasin B. Protein concentration was measured by the modified Bradford protein assay. Cell-associated radioactivity was determined by scintillation counting. The mean counts/min were expressed as pmol/min/mg protein from determinations done in … WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. … how does kelly ripa stay fitWebCytochalasin B (50µM) was added to determine assay background. Glucose Uptake-Glo™ Assay We have developed a luminescent glucose uptake assay that is based on the same principle as the aforementioned … photo of agate stone

"WebCytochalasin B, which is often used as an inhibitor of actin-based motility systems, is also a very potent competitive inhibitor of Đ-glucose uptake into mammalian cells. When red blood cell ghosts (yes it's a real term, these are RBCs emptied of their cytoplasm) are incubated when 'H-cytochalasin and then irradiated with ultraviolet light, the " - Cytochalasin b glucose uptake

Cytochalasin b glucose uptake

Change caused by insulin in the number of cytochalasin B (CB) …

WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release. WebVisfatin stimuli also enhanced intracellular NAD levels, and treatment with FK866, which is a specific inhibitor of nicotinamide phosphoribosyltransferase (Nampt), significantly inhibited visfatin-induced NAD synthesis and glucose uptake. Visfatin treatment increased glucose transporter-1 (GLUT-1) protein expression in isolated cellular ...

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WebMay 13, 2024 · Impairment of cellular glucose uptake due to compromised insulin ... we report the near atomic resolution cryo-EM structures of GLUT4 bound to a small … WebFeb 1, 2013 · RBCs from Atlantic salmon showed a linear relationship between glucose uptake and extracellular glucose level, but exhibited clear inhibition of glucose metabolism by cytochalasin B and phloretin, suggesting a component of facilitated glucose transport that is more elusive to detect.

WebJul 1, 1974 · Cytochalasin B in this concentration suppresses multiplication rates in a nutrient medium consisting of 2 % proteose peptone, but multiplication is unaffected if … WebEnter the email address you signed up with and we'll email you a reset link.

WebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range. Web2.2 Assaying Insulin-Stimulated Glucose Uptake. 1. Serum-free media: DMEM (with 4.5 g/L glucose, l-glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin …

WebMar 1, 1990 · Glucose uptake in muscle occurs by a system of facilitated diffusion involving at least two distinct glucose transporters, GLUT-1 and GLUT-4. Both bind the fungal …

WebMay 23, 2003 · Glucose levels dropped even in the presence of the transport inhibitor cytochalasin B, indicating rapid metabolism. Consistently, the addition of 2 … how does kenrick h20 sandy choreographWebCytochalasin B has been shown to potently inhibit the transport of glucose, deoxyglucose, and glucosamine by Novikoff hepatoma cells in suspension culture without affecting their … photo of agilityWebCytochalasin B, CAS: 14930-96-2, is a cell-permeable fungal toxin used in actin polymerization studies and cytological research.. Cited in 16 publications ... Effect of cytochalasin B on glucose uptake, utilization, oxidation and insulinotropic action in tumoral insulin-producing cells. Malaisse, WJ., et al. 1987. Cell Biochem Funct. 5: 183-7. photo of agaveWebAbstract. Cytochalasin B (17-3 microM) virtually abolished 3-O-methyl-D- [U-14C]glucose uptake and D- [5-3H]glucose utilization in tumoral insulin-producing cells of the RINm5F … how does kelly ripa stay so youngWebMay 13, 2024 · Insulin stimulates glucose uptake by acutely upregulating GLUT4 surface levels mainly through increased exocytosis of GSVs and decreased endocytosis 33, 34. A single GLUT4 molecule during its... how does kelly ripa fix her hairWebMay 1, 1972 · Abstract. A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, … how does kenny keep coming backWebJan 1, 1990 · Cytochalasin B also blocked the uptake of 14 C-L-ascorbic acid (1.25 mg%). The results of these studies further support the hypothesis that glucose and ascorbate … photo of agenda